Cover of: Nuclear Reprogramming | Steve Pells

Nuclear Reprogramming

Methods and Protocols (Methods in Molecular Biology)
  • 348 Pages
  • 1.34 MB
  • 7458 Downloads
  • English
by
Humana Press
Cellular biology, Molecular biology, Science, Cell nuclei, physiology, Science/Mathematics, methods, Biotechnology, Life Sciences - Cytology, Science / Cytology, Cell Nucleus, General, Cell Fusion, Laboratory methods, Transplant
The Physical Object
FormatHardcover
ID Numbers
Open LibraryOL8828475M
ISBN 101588293793
ISBN 139781588293794

Nuclear Reprogramming. Cellular reprogramming is a technique that allows the generation of specific cell types (including stem cell–like cells, neurons, and glia) by genetically modifying readily available somatic Nuclear Reprogramming book such as fibroblasts or lymphocytes to generate patient-specific cell lines.

Cellular and nuclear reprogramming. This note explains the following topics related to Cellular and nuclear reprogramming: Inducing pluripotency, Stem cells in animal models of regeneration, Small RNAs – their biogenesis, regulation and function in embryonic stem cells.

In Nuclear Reprogramming: Methods and Protocols, hands-on laboratory scientists describe readily reproducible methods for actually performing nuclear reprogramming by nuclear transfer in several different species, by fusion induced both chemically and electrically, and by the in vivo treatment of cells with cell extracts.

In Nuclear Reprogramming: Methods and Protocols, hands-on laboratory scientists describe readily reproducible methods for actually performing nuclear reprogramming by nuclear transfer in several different species, by fusion induced both chemically and electrically, and by the in vivo treatment of cells with cell : Hardcover.

Nuclear reprogramming describes a switch in gene expression of one kind of cell to that of another unrelated cell type. Early studies in frog cloning provided some of the first experimental evidence for reprogramming.

Subsequent procedures included mammalian somatic cell nuclear transfer, cell fusion, induction of pluripotency by ectopic gene expression, and direct by:   Nuclear Reprogramming and Stem Cells. Stem Cell Biology and Regenerative Medicine. Share your thoughts Complete your review. Tell readers what you thought by rating and reviewing this book.

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Cellular and nuclear reprogramming. Cellular and nuclear reprogramming. Inducing pluripotency. Kit T Rodolfa. Stem cells in animal models of regeneration. Kyle A. Gurley, Alejandro Sánchez Alvarado. Small RNAs – their biogenesis, regulation and function in embryonic stem cells.

Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, Nuclear Reprogramming book, and epigenetic level.

Over. Nuclear Reprogramming and Stem Cells (Stem Cell Biology and Regenerative Medicine) - Kindle edition by Justin Ainscough, Shinya Yamanaka, Takashi Tada. Download it once and read it on your Kindle device, PC, phones or tablets. Use features like bookmarks, note taking and highlighting while reading Nuclear Reprogramming and Stem Cells (Stem Cell Biology and Regenerative Medicine).

Although nuclear reprogramming-the cellular process underlying cloning and the artificial generation of stem cells from somatic cells-may be considered a "black box" whose interior events are either completely unknown or at least imperfectly characterized, tantalizing clues about its operation are beginning to emerge.

Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level.

Over the past several years, many technical improvements have been made to improve somatic cell nuclear. Nuclear reprogramming, the process by which a nucleus acquires developmental potential, is covered here as well.

It is relevant to stem cell research generally, and also to research on the cloning of animals by nuclear transfer. Nuclear Reprogramming. Hoping to resolve the embryonic-stem-cell debate, Markus Grompe envisions a more ethical way to derive the cells.

Erika Jonietz Monday, Ma This article is the fifth in a series of 10 stories we’re running over two weeks, covering today’s most significant (and just plain cool) emerging technologies.

Reprogramming is distinct from development of a somatic epitype, as somatic epitypes can potentially be altered after an organism has left the developmental stage of life.

During somatic cell nuclear transfer, the oocyte turns off tissue specific genes in the Somatic cell nucleus and turns back on. a| Nuclear transplantation in xenopus / John B. Gurdon -- Nuclear transfer in sheep / William A.

Ritchie -- Protocols for nuclear transfer in mice / Shaorong Gao -- Isolation of stromal stem cells from human adipose tissue / Andrew C. Boquest [et al.] -- Nuclear reprogramming by cell fusion / Robert H. Broyles [et al.] -- Polyethylene glycol-mediated cell fusion / Jian Yang and Ming.

Cellular and nuclear reprogramming - StemBook. Cellular and nuclear reprogramming - StemBook. Your browsing activity is empty. Activity recording is turned off. Turn recording back on. See more Support Center Support Center. External link. Please review our privacy policy. NLM. NIH. In our laboratory we work towards understanding how the cellular reprogramming works at the molecular level.

We use this knowledge to generate isogenic - fully inmune compatible - stem cells, and to improve the efficiency of somatic cell nuclear transfer (SCNT). Introduction by John Gurdon and Azim Surani.- Introduction by Ian Wilmut.- Inherent Nuclear Reprogramming in Mammalian Embryos.- Epigenetic Reprogramming During Somatic Cell Nuclear Transfer and the Development of Primordial Germ Cells.- Epigenetic Reprogramming with Oocyte Molecules.- Cell Fusion-Mediated Nuclear Reprogramming of Somatic Cells Nuclear transplantation in xenopus / John B.

Gurdon --Nuclear transfer in sheep / William A. Ritchie --Protocols for nuclear transfer in mice / Shaorong Gao --Isolation of stromal stem cells from human adipose tissue / Andrew C.

Boquest [and others] --Nuclear reprogramming by cell fusion / Robert H. Broyles [and others] --Polyethylene glycol. In contrast to pluripotent stem cells generated by somatic cell nuclear-transfer (SCNT) or ES cells derived from the inner cell mass (ICM) of the blastocyst, direct reprogramming provides a convenient and reliable means of generating pluripotent stem cells.

iPS cells have already shown incredible potential for research and for therapeutic Cited by: Nuclear reprogramming of somatic cells in hybrid cells is demonstrated by the reactivation of the inactive X chromosome present in a female somatic cell, contribution of the ES and gEG hybrid cells to the primary germ layers of chimeric embryos, and reactivation of the somatic-cell-derived Oct4–GFP marker gene, which is specifically expressed.

include DNA methylation and histone modification that must be reset in reprogramming cells Nuclear Transfer (somatic cell nuclear transfer - SCNT) Involves the physical transplantation of a single nucleus into a meiotic metaphase II arrested egg after removing the recipient egg's genetic material.

Understanding Nuclear Transfer and Stem Cells Mission: We work towards understanding how the reprogramming process works at the molecular level.

We use this knowledge to generate isogenic - fully inmune compatible - stem cells, and to improve the efficiency of somatic cell nuclear transfer (SCNT). It is therefore particularly well-timed of Humana to release Nuclear Reprogramming: Methods and Protocols devoted to research techniques in nuclear reprogramming.

I hope that this book will be of interest to a variety of people, be they cloners interested in the generation of live animals from cells, perhaps endangered species, or medics.

Free Online Library: Biology and Pathology of the Oocyte: Role in Fertility, Medicine, and Nuclear Reprogramming, 2nd Edition.(Brief article, Book review) by "Reference & Research Book News"; Publishing industry Library and information science Books Book reviews. StemBook in conversation with Joanne Kurtzberg about cord blood transplantation, cerebral palsy, and metabolic diseases Foxc1 is a critical regulator of haematopoietic stem/progenitor cell niche formation.

Nuclear Reprogramming in Cells J. Gurdon1 and D. Melton2 Nuclear reprogramming describes a switch in gene expression of one kind of cell to that of another unrelated cell type.

Early studies in frog cloning provided some of the first experimental evidence for reprogramming. Subsequent procedures included mammalian somatic cell nuclear.

Nuclear reprogramming, the process by which a nucleus acquires developmental potential, is currently a focus of great interest in the scientific community and is covered here.

Description Nuclear Reprogramming PDF

It is relevant to stem cell research generally, and also to research on the cloning of animals by nuclear cturer: Wiley. Nuclear transfer.

When a nucleus from a differentiated somatic cell, such as an intestinal cell, is transplanted into an enucleated oocyte, nuclear reprogramming is initiated, leading to the. Nuclear Reprogramming and Stem Cells will serve as a valuable resource for all researchers in the field of stem cell biology, including those just setting out on their career path as well as those already established in the : Humana Press.

Nuclear reprogramming thus provides the means to create invaluable materials for experimental or therapeutic purposes. In the late s, John Gurdon (Cambridge University) transferred nuclei from adult cells into eggs and showed that the resulting cells took on embryonic characteristics.Experimentally, nuclear reprogramming can be achieved by somatic cell nuclear transfer (SCNT) into oocytes, by cell fusion, or by introduction of specific transcription factors into a cell."Symposium on Stem cells: nuclear reprogramming and therapeutic applications, held at the Novartis Foundation, London, March "--p.

v Includes bibliographic references and indexes.

Details Nuclear Reprogramming EPUB

Subject headings Stem cells--Congresses. Stem cells--Therapeutic use--Congresses. Medical subjects Stem Cells--congresses. ISBN.